Researchers continue to investigate the cause of Tutankhamun's premature death. Bob Brier, a mummy specialist from Long Island University, has been tracking down clues that indicate Tutankhamun may have been killed by his elderly chief advisor and successor, Ay. An X-ray of his skull revealed a calcified blood clot at its base. This could have been caused by a blow from a blunt implement, which eventually resulted in death.
The painting in Tutankhamun’s burial chamber depicts Ay at the "opening of the mouth" ceremony, giving life and breath to the young deceased pharaoh. Ay, a commoner, is wearing the leopard skin of a high priest and the crown of a pharaoh. Since Tutankhamun did not have a child to succeed him, it appears that Ay decided to seize the crown and declare himself King of Egypt.
So who did Ankhesenamum marry? There is now evidence that she married Ay. A ring has been found with her cartouche inscribed next to his. Did Ay force her to marry him, thus legitimizing his claim to the throne? Within three years of Ay's death, Ankhesenamum disappeared. Could she also have been the victim of a serial killer?
What happened to Ay? He died within a few years of seizing the throne. His cartouches, which he had inscribed on temple walls, were eradicated, his tomb was robbed and vandalized, and his mummy disappeared. His name was also eliminated from the official list of pharaohs, as was that of Tutankhamun.
Another theory on Tutankhamun's death suggests that he was murdered by General Horemheb, a man of low birth who became one of Akhenaten's closest advisors. Under Tutankhamun, he was appointed commander-in-chief of the army and deputy of the king. Following the demise of Tutankhamun and Ay, Horemheb became pharaoh. During his reign, he had the names of Akhenaten, Tutankhamun and Ay removed from the royal list of pharaohs, which suggests that he had personal reasons for eradicating those rulers from the records.
A new theory contends that the balance of probability is highly in favour of Tutankhamen having died of complications from aninherited genetic disorder rather than at the hands of a murderer.
Since Carter returned Tut to his tomb in 1926, the young king has been x-rayed twice: in 1968 by a team from England's University of Liverpool and in 1978 by a University of Michigan anthropologist.
The 1968 x-rays revealed a bone fragment inside the king's skull. The finding prompted the theory that the boy king was murdered by a blow to the head during the unsettled period of his reign.
In January 2005 the mummy was removed from its tomb in the Valley of the Kings for the first time in almost 80 years. The remains — still lying in the tray of sand where they had been placed by Carter — were taken from the sarcophagus and transported to a nearby trailer with mobile CT scanner. The 15-minute CT-scan session yielded some 1,700 images.
Detailed CT scans of King Tutankhamun's mummy found no physical evidence of murder, according to the announcement made by Egypt's Supreme Council of Antiquities, after the images were analysed. But the scans did reveal unusual features, including a broken leg, which some experts think may have led to the boy king's death.
The scans cannot rule out "non violent" murder, such as poisoning. But they have apparently disproved the oft-repeated theory that King Tutankhamun was murdered by a blow to the head.
The bone fragments revealed by the 1968 x-rays are now deemed to be after-death damage, likely inflicted by Carter, when Carter and his team dismembered much of the mummy to retrieve artifacts and remove the body from its sarcophagus, because the fragments show no evidence of being inundated with the embalming fluid used to preserve the pharaoh for the afterlife. Tutankhamun had been affixed to his coffin by the resins and other fluids used in the embalming process.
The controversy continues. No concrete conclusion has been arrived yet. Did the boy king die a violent or non-violent death? Was he murdered – by a blow on the head or by poison – or did from an accident injury or a clot in the brain arising from a genetic disorder? Will we ever know the truth?